Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1109776..1110338 Replicon chromosome
Accession NZ_CP069549
Organism Escherichia coli strain FDAARGOS_1253

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6J83_RS05250 Protein ID WP_000605675.1
Coordinates 1109776..1110054 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6J83_RS05255 Protein ID WP_000781370.1
Coordinates 1110054..1110338 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J83_RS05225 1105363..1105794 - 432 WP_000152305.1 peroxiredoxin OsmC -
I6J83_RS05230 1106139..1106354 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6J83_RS05235 1106456..1106593 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6J83_RS05240 1106749..1108446 + 1698 WP_000433462.1 malate dehydrogenase -
I6J83_RS05245 1108580..1109590 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
I6J83_RS05250 1109776..1110054 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J83_RS05255 1110054..1110338 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
I6J83_RS05260 1110567..1111220 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6J83_RS05265 1111213..1112097 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
I6J83_RS05270 1112110..1115157 - 3048 WP_077627348.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T190877 WP_000605675.1 NZ_CP069549:1109776-1110054 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T190877 NZ_CP091566:c875786-875511 [Salmonella enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT190877 WP_000781370.1 NZ_CP069549:1110054-1110338 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT190877 NZ_CP091566:c876049-875789 [Salmonella enterica]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References