Detailed information of TA system TA190859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4250400..4251094
• Replicon: chromosome
• Accession: NZ_CP069542
• Organism: Escherichia coli strain FDAARGOS_1248

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: I6J78_RS20485
• Protein ID: WP_001263489.1
• Coordinates: 4250400..4250798 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6J78_RS20490
• Protein ID: WP_000554758.1
• Coordinates: 4250801..4251094 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4245988..4246068	-	81	NuclAT_11	-	-
-	4245988..4246068	-	81	NuclAT_11	-	-
-	4245988..4246068	-	81	NuclAT_11	-	-
-	4245988..4246068	-	81	NuclAT_11	-	-
I6J78_RS20460	4246664..4247122	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
I6J78_RS20465	4247383..4248840	+	1458	WP_204148138.1	cytosol nonspecific dipeptidase	-
I6J78_RS20470	4248897..4249418	-	522	Protein_3986	peptide chain release factor H	-
I6J78_RS20475	4249414..4249620	-	207	Protein_3987	RtcB family protein	-
I6J78_RS20480	4249938..4250390	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
I6J78_RS20485	4250400..4250798	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6J78_RS20490	4250801..4251094	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6J78_RS20495	4251146..4252201	-	1056	WP_158138189.1	DNA polymerase IV	-
I6J78_RS20500	4252272..4253057	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
I6J78_RS20505	4253029..4254741	+	1713	Protein_3993	flagellar biosynthesis protein FlhA	-
I6J78_RS20510	4254965..4255462	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / tssA / hcp1/tssD1	4250400..4278608	28208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T190859 WP_001263489.1 NZ_CP069542:c4250798-4250400 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T190859 NZ_CP091563:c994933-994658 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT190859 WP_000554758.1 NZ_CP069542:c4251094-4250801 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT190859 NZ_CP091563:994668-995090 [Salmonella enterica]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3