Detailed information of TA system TA190828

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 18651..18904
• Replicon: plasmid unnamed2
• Accession: NZ_CP069530
• Organism: Escherichia coli strain FDAARGOS_1252

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: I6J82_RS25430
• Protein ID: WP_021515405.1
• Coordinates: 18755..18904 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	18651..18710 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J82_RS25395	14778..15524	+	747	WP_000205725.1	conjugal transfer pilus acetylase TraX	-
I6J82_RS25400	15579..16139	+	561	WP_001620825.1	fertility inhibition protein FinO	-
I6J82_RS25405	16274..16486	+	213	WP_097424844.1	ANR family transcriptional regulator	-
I6J82_RS25410	16731..17192	+	462	WP_052983101.1	thermonuclease family protein	-
I6J82_RS25415	17238..17447	+	210	WP_097424846.1	hemolysin expression modulator Hha	-
I6J82_RS25420	17485..18171	+	687	WP_001620830.1	DUF2726 domain-containing protein	-
I6J82_RS25425	18168..18476	+	309	WP_001620832.1	hypothetical protein	-
-	18651..18710	-	60	NuclAT_0	-	Antitoxin
-	18651..18710	-	60	NuclAT_0	-	Antitoxin
-	18651..18710	-	60	NuclAT_0	-	Antitoxin
-	18651..18710	-	60	NuclAT_0	-	Antitoxin
I6J82_RS25430	18755..18904	+	150	WP_021515405.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6J82_RS25435	19059..19352	+	294	WP_133258411.1	hypothetical protein	-
I6J82_RS25440	19538..20236	+	699	WP_115766398.1	acid phosphatase AphA	-
I6J82_RS25445	20538..20795	+	258	WP_001620838.1	replication regulatory protein RepA	-
I6J82_RS25450	21029..21103	+	75	WP_001367777.1	RepA leader peptide Tap	-
I6J82_RS25455	21096..21953	+	858	WP_123004306.1	incFII family plasmid replication initiator RepA	-
I6J82_RS25460	22654..22914	+	261	WP_097403166.1	hypothetical protein	-
I6J82_RS25465	23021..23284	+	264	WP_001089474.1	hypothetical protein	-
I6J82_RS25470	23274..23537	+	264	Protein_24	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id	senB / iroN / iroE / iroD / iroC / iroB	1..96693	96693
	-	inside	IScluster/Tn	-	-	23618..28497	4879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5604.75 Da
• Isoelectric Point: 8.7678

>T190828 WP_021515405.1 NZ_CP069530:18755-18904 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVMQVTLAYEARK

Nucleotide

• Length: 150 bp

>T190828 NZ_CP091558:c1241772-1241554 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT190828 NZ_CP069530:c18710-18651 [Escherichia coli]
TAAGTACATCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin