Detailed information of TA system TA190824

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29704..30130
• Replicon: plasmid unnamed1
• Accession: NZ_CP069529
• Organism: Escherichia coli strain FDAARGOS_1252

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J82_RS25195
• Protein ID: WP_001312861.1
• Coordinates: 29704..29862 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	29906..30130 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J82_RS25160	25038..25742	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I6J82_RS25165	25838..26347	+	510	WP_000571065.1	trimethoprim-resistant dihydrofolate reductase DfrA8	-
I6J82_RS25170	26344..27000	+	657	WP_000144779.1	metallophosphoesterase	-
I6J82_RS25175	27037..27741	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I6J82_RS25180	27795..28382	-	588	Protein_18	DUF945 domain-containing protein	-
I6J82_RS25185	28493..28789	-	297	WP_001272251.1	hypothetical protein	-
I6J82_RS25190	29089..29466	+	378	Protein_20	hypothetical protein	-
I6J82_RS25195	29704..29862	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	29906..30130	-	225	NuclAT_0	-	Antitoxin
-	29906..30130	-	225	NuclAT_0	-	Antitoxin
-	29906..30130	-	225	NuclAT_0	-	Antitoxin
-	29906..30130	-	225	NuclAT_0	-	Antitoxin
I6J82_RS25200	30142..30861	-	720	WP_033807739.1	plasmid SOS inhibition protein A	-
I6J82_RS25205	30858..31292	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
I6J82_RS25210	31347..33305	-	1959	WP_058653444.1	ParB/RepB/Spo0J family partition protein	-
I6J82_RS25215	33364..33597	-	234	WP_058653442.1	DUF905 domain-containing protein	-
I6J82_RS25220	33653..34180	-	528	WP_204148107.1	single-stranded DNA-binding protein	-
I6J82_RS25225	34653..34913	-	261	WP_071600428.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA8	-	1..50009	50009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190824 WP_001312861.1 NZ_CP069529:c29862-29704 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190824 NZ_CP091558:360187-360678 [Salmonella enterica]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT190824 NZ_CP069529:c30130-29906 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin