Detailed information of TA system TA190819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4107291..4107893
• Replicon: chromosome
• Accession: NZ_CP069528
• Organism: Escherichia coli strain FDAARGOS_1252

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6J82_RS19725
• Protein ID: WP_000897302.1
• Coordinates: 4107582..4107893 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J82_RS19720
• Protein ID: WP_000356397.1
• Coordinates: 4107291..4107581 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J82_RS19695	4103364..4104266	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6J82_RS19700	4104263..4104898	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6J82_RS19705	4104895..4105824	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6J82_RS19710	4106040..4106258	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
I6J82_RS19715	4106654..4106932	-	279	WP_001296612.1	hypothetical protein	-
I6J82_RS19720	4107291..4107581	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J82_RS19725	4107582..4107893	-	312	WP_000897302.1	hypothetical protein	Toxin
I6J82_RS19730	4108122..4109030	+	909	WP_001331553.1	alpha/beta hydrolase	-
I6J82_RS19735	4109094..4110035	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J82_RS19740	4110080..4110517	-	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J82_RS19745	4110514..4111386	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J82_RS19750	4111380..4111979	-	600	WP_001314324.1	glucose-1-phosphatase	-
I6J82_RS19755	4112078..4112863	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T190819 WP_000897302.1 NZ_CP069528:c4107893-4107582 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T190819 NZ_CP091556:c4141765-4141481 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT190819 WP_000356397.1 NZ_CP069528:c4107581-4107291 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT190819 NZ_CP091556:c4141997-4141755 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: S1P416