Detailed information of TA system TA190808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3044897..3045591
• Replicon: chromosome
• Accession: NZ_CP069528
• Organism: Escherichia coli strain FDAARGOS_1252

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6J82_RS14635
• Protein ID: WP_001263500.1
• Coordinates: 3044897..3045295 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6J82_RS14640
• Protein ID: WP_000554758.1
• Coordinates: 3045298..3045591 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J82_RS14610	3040262..3040720	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
I6J82_RS14615	3040981..3042438	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6J82_RS14620	3042495..3043109	-	615	WP_000602124.1	peptide chain release factor H	-
I6J82_RS14625	3043106..3044245	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
I6J82_RS14630	3044435..3044887	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6J82_RS14635	3044897..3045295	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6J82_RS14640	3045298..3045591	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6J82_RS14645	3045643..3046698	-	1056	WP_001226168.1	DNA polymerase IV	-
I6J82_RS14650	3046769..3047692	-	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
I6J82_RS14655	3047695..3048558	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
I6J82_RS14660	3048571..3049287	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
I6J82_RS14665	3049307..3049774	-	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T190808 WP_001263500.1 NZ_CP069528:c3045295-3044897 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T190808 NZ_CP091556:206805-207290 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT190808 WP_000554758.1 NZ_CP069528:c3045591-3045298 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT190808 NZ_CP091556:206530-206817 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3