Detailed information of TA system TA190806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2818494..2819173
• Replicon: chromosome
• Accession: NZ_CP069528
• Organism: Escherichia coli strain FDAARGOS_1252

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: I6J82_RS13605
• Protein ID: WP_000057523.1
• Coordinates: 2818871..2819173 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: I6J82_RS13600
• Protein ID: WP_000806442.1
• Coordinates: 2818494..2818835 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J82_RS13590	2814738..2815670	-	933	WP_001538399.1	glutaminase A	-
I6J82_RS13595	2815932..2818436	+	2505	WP_000083948.1	copper-exporting P-type ATPase CopA	-
I6J82_RS13600	2818494..2818835	-	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
I6J82_RS13605	2818871..2819173	-	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J82_RS13610	2819306..2820100	+	795	WP_001538398.1	TraB/GumN family protein	-
I6J82_RS13615	2820304..2820783	+	480	WP_001538397.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
I6J82_RS13620	2820820..2822472	-	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
I6J82_RS13625	2822690..2823910	+	1221	Protein_2663	fosmidomycin MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T190806 WP_000057523.1 NZ_CP069528:c2819173-2818871 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T190806 NZ_CP091554:4941337-4941648 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT190806 WP_000806442.1 NZ_CP069528:c2818835-2818494 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT190806 NZ_CP091554:4941649-4941939 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3