Detailed information of TA system TA190788

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43970..44383
• Replicon: plasmid unnamed1
• Accession: NZ_CP069523
• Organism: Escherichia coli strain FDAARGOS_1267

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: I6J97_RS26215
• Protein ID: WP_000775238.1
• Coordinates: 44222..44383 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	43970..44170 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J97_RS26185	39677..40198	+	522	WP_000290849.1	single-stranded DNA-binding protein	-
I6J97_RS26190	40254..40487	+	234	WP_000005971.1	DUF905 domain-containing protein	-
I6J97_RS26195	40548..42497	+	1950	WP_000109422.1	ParB/RepB/Spo0J family partition protein	-
I6J97_RS26200	42552..42986	+	435	WP_000845950.1	conjugation system SOS inhibitor PsiB	-
I6J97_RS26205	42983..43702	+	720	WP_020246013.1	plasmid SOS inhibition protein A	-
I6J97_RS26210	43699..44013	+	315	WP_000870669.1	hypothetical protein	-
-	43970..44170	+	201	NuclAT_0	-	Antitoxin
-	43970..44170	+	201	NuclAT_0	-	Antitoxin
-	43970..44170	+	201	NuclAT_0	-	Antitoxin
-	43970..44170	+	201	NuclAT_0	-	Antitoxin
I6J97_RS26215	44222..44383	+	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6J97_RS26220	44621..44933	-	313	Protein_53	hypothetical protein	-
I6J97_RS26225	45233..45529	+	297	WP_001272251.1	hypothetical protein	-
I6J97_RS26230	45639..46460	+	822	WP_048267032.1	DUF945 domain-containing protein	-
I6J97_RS26235	46757..47359	-	603	WP_077634803.1	transglycosylase SLT domain-containing protein	-
I6J97_RS26240	47680..48063	+	384	WP_001151538.1	relaxosome protein TraM	-
I6J97_RS26245	48250..48939	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67045	67045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T190788 WP_000775238.1 NZ_CP069523:44222-44383 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T190788 NZ_CP091547:c4585156-4584671 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Nucleotide

• Length: 201 bp

>AT190788 NZ_CP069523:43970-44170 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCTA

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin