Detailed information of TA system TA190787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5054462..5055064
• Replicon: chromosome
• Accession: NZ_CP069522
• Organism: Escherichia coli strain FDAARGOS_1267

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A366YL64
• Locus tag: I6J97_RS24480
• Protein ID: WP_001519058.1
• Coordinates: 5054753..5055064 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J97_RS24475
• Protein ID: WP_000356397.1
• Coordinates: 5054462..5054752 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J97_RS24450	5050535..5051437	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6J97_RS24455	5051434..5052069	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6J97_RS24460	5052066..5052995	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6J97_RS24465	5053211..5053429	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
I6J97_RS24470	5053825..5054103	-	279	WP_001296612.1	hypothetical protein	-
I6J97_RS24475	5054462..5054752	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J97_RS24480	5054753..5055064	-	312	WP_001519058.1	hypothetical protein	Toxin
I6J97_RS24485	5055293..5056201	+	909	WP_001331553.1	alpha/beta hydrolase	-
I6J97_RS24490	5056265..5057206	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J97_RS24495	5057251..5057688	-	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J97_RS24500	5057685..5058557	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J97_RS24505	5058551..5059150	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12202.25 Da
• Isoelectric Point: 10.1861

>T190787 WP_001519058.1 NZ_CP069522:c5055064-5054753 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYKIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T190787 NZ_CP091547:c4475236-4474868 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT190787 WP_000356397.1 NZ_CP069522:c5054752-5054462 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT190787 NZ_CP091547:c4475454-4475233 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A366YL64