Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3920025..3920719 Replicon chromosome
Accession NZ_CP069522
Organism Escherichia coli strain FDAARGOS_1267

Toxin (Protein)


Gene name yafO Uniprot ID A0A366YGZ3
Locus tag I6J97_RS18945 Protein ID WP_001518358.1
Coordinates 3920025..3920423 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag I6J97_RS18950 Protein ID WP_000554755.1
Coordinates 3920426..3920719 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J97_RS18920 3915390..3915848 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
I6J97_RS18925 3916109..3917566 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
I6J97_RS18930 3917623..3918237 - 615 WP_000602123.1 peptide chain release factor H -
I6J97_RS18935 3918234..3919373 - 1140 WP_000521578.1 RNA ligase RtcB family protein -
I6J97_RS18940 3919563..3920015 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
I6J97_RS18945 3920025..3920423 - 399 WP_001518358.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
I6J97_RS18950 3920426..3920719 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
I6J97_RS18955 3920771..3921826 - 1056 WP_001226170.1 DNA polymerase IV -
I6J97_RS18960 3921897..3922682 - 786 WP_000207543.1 putative lateral flagellar export/assembly protein LafU -
I6J97_RS18965 3922654..3924366 + 1713 Protein_3721 flagellar biosynthesis protein FlhA -
I6J97_RS18970 3924394..3924888 + 495 WP_139174989.1 hypothetical protein -
I6J97_RS18975 3924883..3925380 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - vat 3861573..3926305 64732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15448.85 Da        Isoelectric Point: 8.0738

>T190778 WP_001518358.1 NZ_CP069522:c3920423-3920025 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMNKIGKMAEAFRMRF

Download         Length: 399 bp

>T190778 NZ_CP091547:583700-583987 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT190778 WP_000554755.1 NZ_CP069522:c3920719-3920426 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT190778 NZ_CP091547:583974-584276 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366YGZ3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References