Detailed information of TA system TA190746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2433200..2433927
• Replicon: chromosome
• Accession: NZ_CP069517
• Organism: Escherichia coli strain FDAARGOS_1262

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: I6J92_RS11610
• Protein ID: WP_000547563.1
• Coordinates: 2433616..2433927 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J92_RS11605
• Protein ID: WP_000126304.1
• Coordinates: 2433200..2433619 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J92_RS11590	2429363..2431615	-	2253	WP_001535518.1	carbamoyltransferase HypF	-
I6J92_RS11595	2431768..2432295	-	528	WP_001078777.1	electron transport protein HydN	-
I6J92_RS11600	2432680..2433108	+	429	WP_000536066.1	DUF4259 domain-containing protein	-
I6J92_RS11605	2433200..2433619	-	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
I6J92_RS11610	2433616..2433927	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6J92_RS11615	2434090..2434845	-	756	WP_001314074.1	hypothetical protein	-
I6J92_RS11620	2434888..2435355	-	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
I6J92_RS11625	2435348..2435758	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6J92_RS11630	2435755..2436522	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6J92_RS11635	2436522..2437064	-	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
I6J92_RS11640	2437074..2438783	-	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2423855..2462762	38907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T190746 WP_000547563.1 NZ_CP069517:c2433927-2433616 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T190746 NZ_CP091542:849435-849833 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT190746 WP_000126304.1 NZ_CP069517:c2433619-2433200 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT190746 NZ_CP091542:849208-849435 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22