Detailed information of TA system TA190721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 90230..90881
• Replicon: plasmid unnamed2
• Accession: NZ_CP069506
• Organism: Acinetobacter pittii strain FDAARGOS_1215

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6J45_RS18110
• Protein ID: WP_000269910.1
• Coordinates: 90573..90881 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J45_RS18105
• Protein ID: WP_001140619.1
• Coordinates: 90230..90532 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J45_RS18070	85736..86056	-	321	WP_001081101.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
I6J45_RS18075	86299..86827	-	529	Protein_82	IS3 family transposase	-
I6J45_RS18080	87032..87715	-	684	WP_000478902.1	hypothetical protein	-
I6J45_RS18085	88273..88629	+	357	WP_000269908.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J45_RS18090	88622..88925	+	304	Protein_85	helix-turn-helix transcriptional regulator	-
I6J45_RS18095	89691..89918	+	228	WP_000555567.1	hypothetical protein	-
I6J45_RS18100	90028..90237	-	210	WP_000069470.1	hypothetical protein	-
I6J45_RS18105	90230..90532	-	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
I6J45_RS18110	90573..90881	-	309	WP_000269910.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J45_RS18115	91896..92012	-	117	Protein_90	transposase	-
I6J45_RS18120	92199..92492	-	294	WP_000221360.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J45_RS18125	92482..92727	-	246	WP_000246757.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
I6J45_RS18130	92984..93886	-	903	WP_000466974.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94379	94379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-102)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11742.64 Da
• Isoelectric Point: 9.0002

>T190721 WP_000269910.1 NZ_CP069506:c90881-90573 [Acinetobacter pittii]
MWTVITTDLFNEWLVQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIAD

Nucleotide

• Length: 309 bp

>T190721 NZ_CP091534:c4491568-4491395 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT190721 WP_001140619.1 NZ_CP069506:c90532-90230 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT190721 NZ_CP091534:c4491892-4491596 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG