Detailed information of TA system TA190720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 56828..57431
• Replicon: plasmid unnamed2
• Accession: NZ_CP069506
• Organism: Acinetobacter pittii strain FDAARGOS_1215

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6J45_RS17970
• Protein ID: WP_000288004.1
• Coordinates: 57102..57431 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J45_RS17965
• Protein ID: WP_001258993.1
• Coordinates: 56828..57115 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J45_RS17920	52263..52718	+	456	WP_001136766.1	hypothetical protein	-
I6J45_RS17925	52779..53240	+	462	WP_000378528.1	hypothetical protein	-
I6J45_RS17930	53209..53850	+	642	WP_000372120.1	hypothetical protein	-
I6J45_RS17935	53941..54464	+	524	Protein_54	DUF2280 domain-containing protein	-
I6J45_RS17940	54448..54957	+	510	Protein_55	terminase	-
I6J45_RS17945	55312..55635	+	324	WP_005804845.1	hypothetical protein	-
I6J45_RS17950	55707..55883	+	177	WP_002120129.1	hypothetical protein	-
I6J45_RS17955	55892..56215	+	324	Protein_58	DUF4236 domain-containing protein	-
I6J45_RS17960	56603..56791	+	189	WP_000340067.1	hypothetical protein	-
I6J45_RS17965	56828..57115	-	288	WP_001258993.1	helix-turn-helix domain-containing protein	Antitoxin
I6J45_RS17970	57102..57431	-	330	WP_000288004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J45_RS17975	57486..57755	-	270	WP_000453245.1	transcriptional regulator	-
I6J45_RS17980	57962..58564	+	603	WP_000096286.1	hypothetical protein	-
I6J45_RS17985	58621..58839	+	219	WP_000994925.1	hypothetical protein	-
I6J45_RS17990	58932..59129	+	198	WP_025464206.1	hypothetical protein	-
I6J45_RS17995	59260..60192	-	933	WP_000743263.1	IS5 family transposase	-
I6J45_RS18000	60359..60544	+	186	WP_000755724.1	hypothetical protein	-
I6J45_RS18005	60547..61059	+	513	WP_000464612.1	Fic family protein	-
I6J45_RS18010	61301..61629	-	329	Protein_69	DUF4236 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94379	94379
	-	flank	IS/Tn	-	-	59260..60192	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12571.69 Da
• Isoelectric Point: 8.8102

>T190720 WP_000288004.1 NZ_CP069506:c57431-57102 [Acinetobacter pittii]
MYTIEYYSEDVQDEILTLPDGLLARYFNLTDRMVIYGANLGEPHTLPMSKGLFELRLKSQEGIARVMYCTLVGKRIVMLH
SFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 330 bp

>T190720 NZ_CP091534:c4387167-4386817 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10565.30 Da
• Isoelectric Point: 10.1453

>AT190720 WP_001258993.1 NZ_CP069506:c57115-56828 [Acinetobacter pittii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTARRDAGLSQAQIAERMGTSVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT190720 NZ_CP091534:c4387412-4387161 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA