Detailed information of TA system TA190719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3652..4303
• Replicon: plasmid unnamed1
• Accession: NZ_CP069505
• Organism: Acinetobacter pittii strain FDAARGOS_1215

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A062G190
• Locus tag: I6J45_RS17630
• Protein ID: WP_005065055.1
• Coordinates: 3959..4303 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J45_RS17625
• Protein ID: WP_005065057.1
• Coordinates: 3652..3954 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J45_RS17605	1757..2044	+	288	WP_002124950.1	BrnT family toxin	-
I6J45_RS17610	2031..2342	+	312	WP_005065064.1	BrnA antitoxin family protein	-
I6J45_RS17615	2433..3107	-	675	WP_005065062.1	HEPN domain-containing protein	-
I6J45_RS17620	3159..3560	-	402	WP_005065059.1	hypothetical protein	-
I6J45_RS17625	3652..3954	-	303	WP_005065057.1	XRE family transcriptional regulator	Antitoxin
I6J45_RS17630	3959..4303	-	345	WP_005065055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J45_RS17635	4824..5141	-	318	WP_000900860.1	hypothetical protein	-
I6J45_RS17640	5491..6021	-	531	WP_005065050.1	plasmid replication DNA-binding protein	-
I6J45_RS17645	6100..7029	-	930	WP_005065049.1	replication initiation protein RepM	-
I6J45_RS17650	7727..8440	-	714	WP_005065071.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11158	11158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13143.23 Da
• Isoelectric Point: 6.4651

>T190719 WP_005065055.1 NZ_CP069505:c4303-3959 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSGKKRFYTEMLAIADKQYELHLSTLGE

Nucleotide

• Length: 345 bp

>T190719 NZ_CP091534:3689036-3689254 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11280.08 Da
• Isoelectric Point: 5.8961

>AT190719 WP_005065057.1 NZ_CP069505:c3954-3652 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT190719 NZ_CP091534:3688636-3689010 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A062G190