Detailed information of TA system TA190713

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24724..25150
• Replicon: plasmid unnamed1
• Accession: NZ_CP069502
• Organism: Escherichia coli strain FDAARGOS_1268

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J98_RS23525
• Protein ID: WP_001312861.1
• Coordinates: 24724..24882 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	24926..25150 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J98_RS23490	19773..20000	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
I6J98_RS23495	20094..20780	-	687	WP_001825184.1	PAS domain-containing protein	-
I6J98_RS23500	20971..21354	-	384	WP_000124981.1	relaxosome protein TraM	-
I6J98_RS23505	21631..22278	+	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
I6J98_RS23510	22575..23396	-	822	WP_001234469.1	DUF945 domain-containing protein	-
I6J98_RS23515	23515..23802	-	288	WP_000107535.1	hypothetical protein	-
I6J98_RS23520	23827..24033	-	207	WP_000547971.1	hypothetical protein	-
I6J98_RS23525	24724..24882	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	24926..25150	-	225	NuclAT_0	-	Antitoxin
-	24926..25150	-	225	NuclAT_0	-	Antitoxin
-	24926..25150	-	225	NuclAT_0	-	Antitoxin
-	24926..25150	-	225	NuclAT_0	-	Antitoxin
I6J98_RS23530	24962..25150	+	189	WP_001299721.1	hypothetical protein	-
I6J98_RS23535	25162..25881	-	720	WP_060588692.1	plasmid SOS inhibition protein A	-
I6J98_RS23540	25878..26312	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6J98_RS23545	26367..28325	-	1959	WP_204088937.1	ParB/RepB/Spo0J family partition protein	-
I6J98_RS23550	28390..28623	-	234	WP_000006030.1	DUF905 family protein	-
I6J98_RS23555	28685..29224	-	540	WP_000290812.1	single-stranded DNA-binding protein	-
I6J98_RS23560	29250..29456	-	207	WP_000547971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..77233	77233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190713 WP_001312861.1 NZ_CP069502:c24882-24724 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190713 NZ_CP091534:c1949365-1948991 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT190713 NZ_CP069502:c25150-24926 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin