Detailed information of TA system TA190710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4139940..4140739
• Replicon: chromosome
• Accession: NZ_CP069501
• Organism: Escherichia coli strain FDAARGOS_1268

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: I6J98_RS19630
• Protein ID: WP_000347273.1
• Coordinates: 4140275..4140739 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: I6J98_RS19625
• Protein ID: WP_001308975.1
• Coordinates: 4139940..4140275 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J98_RS19610	4135725..4136495	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
I6J98_RS19615	4136511..4137845	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
I6J98_RS19620	4138220..4139791	+	1572	WP_204088913.1	galactarate dehydratase	-
I6J98_RS19625	4139940..4140275	+	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I6J98_RS19630	4140275..4140739	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I6J98_RS19635	4140794..4141603	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I6J98_RS19640	4141852..4143132	+	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I6J98_RS19645	4143155..4143628	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I6J98_RS19650	4143639..4144418	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
I6J98_RS19655	4144408..4145286	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
I6J98_RS19660	4145304..4145738	+	435	WP_000948834.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4129459..4140739	11280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T190710 WP_000347273.1 NZ_CP069501:4140275-4140739 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T190710 NZ_CP091534:1439357-1439755 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT190710 WP_001308975.1 NZ_CP069501:4139940-4140275 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT190710 NZ_CP091534:1439130-1439357 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08