Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1618396..1619123 Replicon chromosome
Accession NZ_CP069500
Organism Escherichia coli strain FDAARGOS_1258

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag I6J88_RS08245 Protein ID WP_000547563.1
Coordinates 1618812..1619123 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6J88_RS08240 Protein ID WP_000126304.1
Coordinates 1618396..1618815 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J88_RS08225 1614559..1616811 - 2253 WP_001535518.1 carbamoyltransferase HypF -
I6J88_RS08230 1616964..1617491 - 528 WP_001078777.1 electron transport protein HydN -
I6J88_RS08235 1617876..1618304 + 429 WP_000536066.1 DUF4259 domain-containing protein -
I6J88_RS08240 1618396..1618815 - 420 WP_000126304.1 helix-turn-helix domain-containing protein Antitoxin
I6J88_RS08245 1618812..1619123 - 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
I6J88_RS08250 1619286..1620041 - 756 WP_001314074.1 hypothetical protein -
I6J88_RS08255 1620084..1620551 - 468 WP_000132955.1 hydrogenase maturation peptidase HycI -
I6J88_RS08260 1620544..1620954 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
I6J88_RS08265 1620951..1621718 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
I6J88_RS08270 1621718..1622260 - 543 WP_000493800.1 formate hydrogenlyase subunit HycF -
I6J88_RS08275 1622270..1623979 - 1710 WP_001288123.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1609051..1647969 38918


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T190677 WP_000547563.1 NZ_CP069500:c1619123-1618812 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T190677 NZ_CP091523:2400503-2400610 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15474.50 Da        Isoelectric Point: 4.4596

>AT190677 WP_000126304.1 NZ_CP069500:c1618815-1618396 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT190677 NZ_CP091523:c2400687-2400627 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure

References