Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 925553..926055 Replicon chromosome
Accession NZ_CP069500
Organism Escherichia coli strain FDAARGOS_1258

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I6J88_RS05105 Protein ID WP_000767829.1
Coordinates 925553..925807 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6J88_RS05110 Protein ID WP_001259255.1
Coordinates 925804..926055 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J88_RS05075 920568..920795 - 228 WP_000985267.1 sulfurtransferase TusA family protein -
I6J88_RS05080 920809..921867 - 1059 WP_000492321.1 transport protein YeeE -
I6J88_RS05085 922046..923404 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I6J88_RS05090 923394..923456 - 63 WP_010723108.1 membrane protein YoeI -
I6J88_RS05095 923671..924600 - 930 WP_000803351.1 LysR family transcriptional regulator -
I6J88_RS05100 924646..925470 - 825 WP_000754734.1 SDR family oxidoreductase -
I6J88_RS05105 925553..925807 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6J88_RS05110 925804..926055 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6J88_RS05115 926338..926388 + 51 WP_001364200.1 his operon leader peptide -
I6J88_RS05120 926534..927433 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6J88_RS05125 927439..928743 + 1305 WP_001468360.1 histidinol dehydrogenase -
I6J88_RS05130 928740..929810 + 1071 WP_001099222.1 histidinol-phosphate transaminase -
I6J88_RS05135 929810..930877 + 1068 WP_000080068.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T190676 WP_000767829.1 NZ_CP069500:c925807-925553 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T190676 NZ_CP091523:2369231-2369497 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT190676 WP_001259255.1 NZ_CP069500:c926055-925804 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT190676 NZ_CP091523:2368974-2369231 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References