Detailed information of TA system TA190663

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 115275..115528
• Replicon: plasmid unnamed
• Accession: NZ_CP069499
• Organism: Escherichia coli strain FDAARGOS_1258

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: I6J88_RS00635
• Protein ID: WP_001336447.1
• Coordinates: 115275..115424 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	115472..115528 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J88_RS00595	110708..110887	-	180	Protein_118	entry exclusion protein 1	-
I6J88_RS00600	111079..111726	+	648	WP_021515401.1	hypothetical protein	-
I6J88_RS00605	111951..112250	-	300	WP_021515402.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J88_RS00610	112240..112503	-	264	WP_001089474.1	hypothetical protein	-
I6J88_RS00615	112610..112870	-	261	WP_019842130.1	hypothetical protein	-
I6J88_RS00620	113573..114430	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
I6J88_RS00625	114423..114497	-	75	WP_001365705.1	RepA leader peptide Tap	-
I6J88_RS00630	114733..114990	-	258	WP_000084404.1	replication regulatory protein RepA	-
I6J88_RS00635	115275..115424	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	115472..115528	+	57	NuclAT_1	-	Antitoxin
-	115472..115528	+	57	NuclAT_1	-	Antitoxin
-	115472..115528	+	57	NuclAT_1	-	Antitoxin
-	115472..115528	+	57	NuclAT_1	-	Antitoxin
I6J88_RS00640	115623..116060	-	438	WP_000872609.1	hypothetical protein	-
I6J88_RS00645	116213..116597	-	385	Protein_128	hypothetical protein	-
I6J88_RS00650	116700..117161	-	462	WP_019842128.1	thermonuclease family protein	-
I6J88_RS00655	117903..118106	-	204	WP_001336517.1	hypothetical protein	-
I6J88_RS00660	118258..118815	-	558	WP_000139329.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..118909	118909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T190663 WP_001336447.1 NZ_CP069499:c115424-115275 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T190663 NZ_CP091519:c1610164-1609868 [Alysiella crassa]
ATGAGCTATGAACTGGCATTTTACCCATCAGCACAGCGCGAATGGGATAAATTGGACAACAGCATTAAGCAACAATTTAA
AAATGTGTTGGCAAGGCGTTTGGAAAATCCGCGTGTGCCATCTGCTGCTTTGCACAATATGCCTGATTGCTACAAAATTA
AATTGCGCCGCGCAGGTTATCGTTTGGTGTATCATGTTGATGATAACGTGATTGTGGTTAGCGTGATTTCGGTTGGCAAA
CGTGAACGCAATGCGGTATACGATACCGCGCAAAATCGTTTGCATAATGATGAGTGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT190663 NZ_CP069499:115472-115528 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin