Detailed information of TA system TA190660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 115308..115770
• Replicon: plasmid unnamed2
• Accession: NZ_CP069498
• Organism: Acinetobacter pittii strain FDAARGOS_1217

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: I6J47_RS19045
• Protein ID: WP_000033474.1
• Coordinates: 115308..115496 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J47_RS19050
• Protein ID: WP_001258992.1
• Coordinates: 115483..115770 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J47_RS19005	110469..111005	-	537	WP_009501148.1	porin family protein	-
I6J47_RS19010	111713..112069	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J47_RS19015	112062..112364	+	303	WP_001140619.1	XRE family transcriptional regulator	-
I6J47_RS19020	112357..112566	+	210	WP_000069471.1	hypothetical protein	-
I6J47_RS19025	112691..112903	-	213	WP_016653394.1	hypothetical protein	-
I6J47_RS19030	113662..114207	-	546	Protein_129	IS5 family transposase	-
I6J47_RS19035	114135..114523	-	389	Protein_130	AAA family ATPase	-
I6J47_RS19040	114711..115031	+	321	WP_004704395.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
I6J47_RS19045	115308..115496	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J47_RS19050	115483..115770	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
I6J47_RS19055	116054..116278	-	225	WP_005130229.1	hypothetical protein	-
I6J47_RS19060	116302..116859	-	558	Protein_135	hypothetical protein	-
I6J47_RS19065	116899..118215	-	1317	WP_032042750.1	terminase	-
I6J47_RS19070	118199..118740	-	542	Protein_137	DUF2280 domain-containing protein	-
I6J47_RS19075	118800..119441	-	642	WP_021511117.1	hypothetical protein	-
I6J47_RS19080	119410..119877	-	468	WP_204087067.1	hypothetical protein	-
I6J47_RS19085	119938..120393	-	456	WP_057076637.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128321	128321
	-	inside	IScluster/Tn	-	-	93238..114144	20906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T190660 WP_000033474.1 NZ_CP069498:115308-115496 [Acinetobacter pittii]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T190660 NZ_CP091519:c135209-134934 [Alysiella crassa]
ATGTATGGTATTACTTTTGAACTAGACAAATCCGATTTGCAAATGTTTTACAGTCAAGGTGCGTCTGAAGCCTATGCGGA
AATTGCCCAAATTTTGCAACCCTTTGGTTTTACAGGCGTGGGCAATACTTATTTGTGTAAACAAGGCAATTTGGCGAATA
TGATGGCGGCGGTGGAAGCCTTGAAAAAGCAATCGTGGTTTTCGTCTTCTGTGAGAAATATGGAAACTTTCCGCGTGGCG
GAATGGGTAGATTTGACCGATTTTGTGCGTGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT190660 WP_001258992.1 NZ_CP069498:115483-115770 [Acinetobacter pittii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT190660 NZ_CP091519:c134843-134652 [Alysiella crassa]
ATGATAAATCAAGATAATATCCCAACTGGCGAAGTGCGAACTTTTGGCGCATTCGGTGTGCCTTATTTGGTTGGCAAAGC
GGAACAGGAATTGCCCAATGGCGACATTTTGGTGGGCATTGAATTGTTGGAAAGTGGCGAACATGAACAATATCCGCTCT
CCCATTTGTTGCAAGACCCGAAAGCCAATTAA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35