Detailed information of TA system TA190659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 111713..112364
• Replicon: plasmid unnamed2
• Accession: NZ_CP069498
• Organism: Acinetobacter pittii strain FDAARGOS_1217

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S3SZA7
• Locus tag: I6J47_RS19010
• Protein ID: WP_000269903.1
• Coordinates: 111713..112069 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J47_RS19015
• Protein ID: WP_001140619.1
• Coordinates: 112062..112364 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J47_RS18980	107552..107854	+	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J47_RS18985	107855..108136	+	282	WP_000985610.1	putative addiction module antidote protein	-
I6J47_RS18990	108364..109224	+	861	WP_002009063.1	IS5 family transposase	-
I6J47_RS18995	109361..109732	-	372	WP_009501156.1	RcnB family protein	-
I6J47_RS19000	110069..110299	+	231	WP_009501150.1	hypothetical protein	-
I6J47_RS19005	110469..111005	-	537	WP_009501148.1	porin family protein	-
I6J47_RS19010	111713..112069	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J47_RS19015	112062..112364	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
I6J47_RS19020	112357..112566	+	210	WP_000069471.1	hypothetical protein	-
I6J47_RS19025	112691..112903	-	213	WP_016653394.1	hypothetical protein	-
I6J47_RS19030	113662..114207	-	546	Protein_129	IS5 family transposase	-
I6J47_RS19035	114135..114523	-	389	Protein_130	AAA family ATPase	-
I6J47_RS19040	114711..115031	+	321	WP_004704395.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
I6J47_RS19045	115308..115496	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J47_RS19050	115483..115770	+	288	WP_001258992.1	helix-turn-helix transcriptional regulator	-
I6J47_RS19055	116054..116278	-	225	WP_005130229.1	hypothetical protein	-
I6J47_RS19060	116302..116859	-	558	Protein_135	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128321	128321
	-	inside	IScluster/Tn	-	-	93238..114144	20906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13486.45 Da
• Isoelectric Point: 7.1645

>T190659 WP_000269903.1 NZ_CP069498:111713-112069 [Acinetobacter pittii]
MWTVITTDLFNEWLAQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYEIHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T190659 NZ_CP091519:c134648-134397 [Alysiella crassa]
ATGTATGCCATTTCATTTGATTTAGTTGTTTCCGACACCCAAAATGCCCACCCAAAAGGCGCAACCCTACATCAATACGG
TTTTCAGCGCGTTCAAGGCAGCCTGTATGTCAATGACAATGAAGACATGGCAAACCTGTTTTCTGCCATTCAAGCCTTGA
AAAGTTTGGCGTGGTTTCCCACTTCTGTGCGTGATATTCGCGCATTCCGCATTGAGCAATGGTCGGATTTTACGAAGTTT
GTGAAAACCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT190659 WP_001140619.1 NZ_CP069498:112062-112364 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT190659 NZ_CP091519:c134843-134652 [Alysiella crassa]
ATGATAAATCAAGATAATATCCCAACTGGCGAAGTGCGAACTTTTGGCGCATTCGGTGTGCCTTATTTGGTTGGCAAAGC
GGAACAGGAATTGCCCAATGGCGACATTTTGGTGGGCATTGAATTGTTGGAAAGTGGCGAACATGAACAATATCCGCTCT
CCCATTTGTTGCAAGACCCGAAAGCCAATTAA

Structures

Toxin

• AlphaFold DB: A0A836MG83