Detailed information of TA system TA190649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4394763..4395365
• Replicon: chromosome
• Accession: NZ_CP069488
• Organism: Escherichia coli strain FDAARGOS_1264

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6J94_RS21315
• Protein ID: WP_000897302.1
• Coordinates: 4395054..4395365 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J94_RS21310
• Protein ID: WP_000356397.1
• Coordinates: 4394763..4395053 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J94_RS21280	4390070..4390999	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
I6J94_RS21285	4391181..4391423	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
I6J94_RS21290	4391713..4392561	+	849	WP_001038650.1	hypothetical protein	-
I6J94_RS21295	4392877..4393326	+	450	WP_204097967.1	hypothetical protein	-
I6J94_RS21300	4393511..4393729	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
I6J94_RS21305	4394126..4394404	-	279	WP_001296612.1	hypothetical protein	-
I6J94_RS21310	4394763..4395053	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J94_RS21315	4395054..4395365	-	312	WP_000897302.1	hypothetical protein	Toxin
I6J94_RS21320	4395594..4396502	+	909	WP_001385591.1	alpha/beta hydrolase	-
I6J94_RS21325	4396566..4397507	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J94_RS21330	4397552..4397989	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J94_RS21335	4397986..4398858	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J94_RS21340	4398852..4399451	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T190649 WP_000897302.1 NZ_CP069488:c4395365-4395054 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T190649 NZ_CP091514:664-918 [Vitreoscilla stercoraria]
ATGAAACGAAAACATCAAAAAACCTTAGAACTGATTTTCCATCGACCAGTATCAGGAAGTATAAAATGGACGGAGATCGA
AGCCTTATTTATTGAGCTAGGAGCAAACGTGCAGGAGCGTGAAGGTTCACGTATAGCGGTTGTGTTATTCGGACAGGTAA
AAGTGTTTCATCGTCCGCATCCTAGCCCTGATACCGATAAAGGGGCTGTTGCCAGTGTTCGAAAGTGGTTAGATGAAAAT
GGAGTAAGACCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT190649 WP_000356397.1 NZ_CP069488:c4395053-4394763 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT190649 NZ_CP091514:915-1250 [Vitreoscilla stercoraria]
ATGATTAATACGATGATAATTAATGGCTACAAAGCCGTGATTGCATATGATTCTGAAATTGAGTTGTTTCGCGGTGAATT
TATTGGATTAAATGGTGGAGCAGATTTTTATGCGGCTGATGTAGAAAGCTTACGTAGAGAAGGTGCTGAATCATTAAAAG
TGTTTTTAGAAGTGTGTAAAGAGAACGGTATCGAGCCAAAAAAACAATATTCAGGACGCTTTAATGTTCGGATTAATCCG
AAATTACATGAAGATGCCATGGCTGCTGCTATTGCTCAAGGCATCAGCCTAAATGAGTTAGTTAGCCGTGCCATTGCCAC
AGCAGCACATCATTAA

Structures

Toxin

• AlphaFold DB: S1P416