Detailed information of TA system TA190640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3188744..3189423
• Replicon: chromosome
• Accession: NZ_CP069488
• Organism: Escherichia coli strain FDAARGOS_1264

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: I6J94_RS15665
• Protein ID: WP_000057523.1
• Coordinates: 3189121..3189423 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: I6J94_RS15660
• Protein ID: WP_000806442.1
• Coordinates: 3188744..3189085 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J94_RS15650	3184988..3185920	-	933	WP_000883041.1	glutaminase A	-
I6J94_RS15655	3186182..3188686	+	2505	WP_000083947.1	copper-exporting P-type ATPase CopA	-
I6J94_RS15660	3188744..3189085	-	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
I6J94_RS15665	3189121..3189423	-	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J94_RS15670	3189556..3190350	+	795	WP_204097949.1	TraB/GumN family protein	-
I6J94_RS15675	3190554..3191033	+	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
I6J94_RS15680	3191057..3191857	+	801	WP_000439798.1	hypothetical protein	-
I6J94_RS15685	3191854..3192357	+	504	WP_000667000.1	hypothetical protein	-
I6J94_RS15690	3192395..3194047	-	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T190640 WP_000057523.1 NZ_CP069488:c3189423-3189121 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T190640 NZ_CP091508:2033052-2033234 [Uruburuella testudinis]
ATGAAATACAGTGAATTTCGAAAGTGGCTTTTATCTCAAGGTGTGGAATTTACCACACAAAAGCGCGGCAGCCATCAGTT
AATCAGGTTGGGCAACAAAACATCGGTTTTCCCGAACCACGGCAGTAAAGAAATCGGTACGGGGCTGGTCAACAAAATCA
AGAAAGATTTAGGTCTGAAATAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT190640 WP_000806442.1 NZ_CP069488:c3189085-3188744 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT190640 NZ_CP091508:2033279-2033698 [Uruburuella testudinis]
ATGTTAGCTTATCCCTACAAACTAACCCCCGACGACAACGGCACTTTTTTGGTAACTTTTCCTGACATTCCCGAAGCGAC
CGCTGTTGGTGAAGACTTGGAATCGGCCAAAATCGAAGCCTTAGACGGCCTGCTGTGTGCATTAGACGGTTATTTCGAAG
ACCGCCGAGCCATCCCCTTACCGGGCAGCTACAGCGATACACAAGACACCGTCAGCCTGCCGGCATTAGAAACCGCCAAA
GTGCTGCTGCTGAATGAAATGGTCAGCCAGGGCGTAAAGAAAGCAGAAATGGCGCGGCGGCTCGAAGTGCATATGCCGCA
AGTTGACCGCCTGCTTGATTTGCGCCACAACACCAAAATCGATTTTATGGAAAAAGCCGCTTCAAAACTGGGGAAGCATC
TGAATATCAGCTTTTCTTAA

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3