Detailed information of TA system TA190627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1498280..1498782
• Replicon: chromosome
• Accession: NZ_CP069488
• Organism: Escherichia coli strain FDAARGOS_1264

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: I6J94_RS07045
• Protein ID: WP_000767819.1
• Coordinates: 1498528..1498782 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: I6J94_RS07040
• Protein ID: WP_001259253.1
• Coordinates: 1498280..1498531 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J94_RS07015	1493458..1494525	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
I6J94_RS07020	1494525..1495595	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
I6J94_RS07025	1495592..1496896	-	1305	WP_001296211.1	histidinol dehydrogenase	-
I6J94_RS07030	1496902..1497801	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
I6J94_RS07035	1497947..1497997	-	51	WP_001364200.1	his operon leader peptide	-
I6J94_RS07040	1498280..1498531	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
I6J94_RS07045	1498528..1498782	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
I6J94_RS07050	1498865..1499689	+	825	WP_000754737.1	SDR family oxidoreductase	-
I6J94_RS07055	1499735..1500664	+	930	WP_000803366.1	LysR family transcriptional regulator	-
I6J94_RS07060	1500879..1500941	+	63	WP_010723108.1	membrane protein YoeI	-
I6J94_RS07065	1500931..1502289	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
I6J94_RS07070	1502506..1502964	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1502506..1502964	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T190627 WP_000767819.1 NZ_CP069488:1498528-1498782 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T190627 NZ_CP091494:c58265-57978 [Enterobacter cloacae complex sp. ECL405]
ATGGCATATTTTCTGGATTTTGACGAGCGTGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGCTAAA
AAATAAGTTGGCTGAAGTGCTTGAGTCACCCCGAATTGATGCGAACAAGCTTCGGGGTATGCATGACTGTTACAAAATTA
AGCTCCGGTCTTCAGGCTATCGTCTTGTATATCAGGTCATAGACGAGAAGGTTGTCGTCTTTGTGATTTCTGTCGGGAAA
AGAGAGCGCTCGGAAGTTTATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT190627 WP_001259253.1 NZ_CP069488:1498280-1498531 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT190627 NZ_CP091494:c58504-58265 [Enterobacter cloacae complex sp. ECL405]
ATGGGTAGCATCAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCTGCGCTTGAAAAAATGGGTGTAACTCC
CTCTGAGGCACTTCGCCTCATGCTTGAATATATTGCTGACAATGAACGTTTGCCGTTCAAACAGACACTCCTGAGTGATG
AGGATGCTGAGCTCGTGGAGATCGTGAAAGAACGACTTCGCAATCCTAAGCCAGTACGTGTGACGCTGGATGAGCTCTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3