Detailed information of TA system TA190599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3185709..3186508
• Replicon: chromosome
• Accession: NZ_CP069480
• Organism: Escherichia coli strain FDAARGOS_1257

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: I6J87_RS15390
• Protein ID: WP_000347252.1
• Coordinates: 3185709..3186173 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: I6J87_RS15395
• Protein ID: WP_001296435.1
• Coordinates: 3186173..3186508 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J87_RS15360	3180710..3181144	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
I6J87_RS15365	3181162..3182040	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
I6J87_RS15370	3182030..3182809	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
I6J87_RS15375	3182820..3183293	-	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I6J87_RS15380	3183316..3184596	-	1281	WP_000681930.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I6J87_RS15385	3184845..3185654	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I6J87_RS15390	3185709..3186173	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I6J87_RS15395	3186173..3186508	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I6J87_RS15400	3186657..3188228	-	1572	WP_001273939.1	galactarate dehydratase	-
I6J87_RS15405	3188603..3189937	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
I6J87_RS15410	3189953..3190723	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T190599 WP_000347252.1 NZ_CP069480:c3186173-3185709 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T190599 NZ_CP091482:94125-95450 [Enterobacter cloacae complex sp. ECL414]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT190599 WP_001296435.1 NZ_CP069480:c3186508-3186173 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT190599 NZ_CP091482:93827-94120 [Enterobacter cloacae complex sp. ECL414]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5