Detailed information of TA system TA190596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2350622..2351224
• Replicon: chromosome
• Accession: NZ_CP069480
• Organism: Escherichia coli strain FDAARGOS_1257

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6J87_RS11380
• Protein ID: WP_000897305.1
• Coordinates: 2350913..2351224 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J87_RS11375
• Protein ID: WP_000356397.1
• Coordinates: 2350622..2350912 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J87_RS11355	2347124..2348026	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6J87_RS11360	2348023..2348658	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6J87_RS11365	2348655..2349584	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6J87_RS11370	2349799..2350017	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
I6J87_RS11375	2350622..2350912	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J87_RS11380	2350913..2351224	-	312	WP_000897305.1	hypothetical protein	Toxin
I6J87_RS11385	2351453..2352361	+	909	WP_001298596.1	alpha/beta hydrolase	-
I6J87_RS11390	2352425..2353366	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J87_RS11395	2353411..2353848	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J87_RS11400	2353845..2354717	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J87_RS11405	2354711..2355310	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T190596 WP_000897305.1 NZ_CP069480:c2351224-2350913 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T190596 NZ_CP091481:c3885396-3884986 [Enterobacter cloacae complex sp. ECL414]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCGTTAATGCTCCACGGCCTGGTTGCGGC
CATCGTGTTACTCATGCCATGGCCCCTGAGCTATACACCGCTGTGGCTGCTGTTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCTCGTCAGGGTGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTGGGTATGCCATGGATGCTCAACTCCGGCATGATGTTACGGTTACGCAATGTGGACGGCGGGCGTTG
CCAGCACCTGTGGCTGGCGGCTGACAGTATGGATGCTTCAGAATGGCGAGATCTGCGCCGGATGTTGTTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT190596 WP_000356397.1 NZ_CP069480:c2350912-2350622 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT190596 NZ_CP091481:c3885643-3885377 [Enterobacter cloacae complex sp. ECL414]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGGCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7