Detailed information of TA system TA190589

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 1695197..1695609
• Replicon: chromosome
• Accession: NZ_CP069480
• Organism: Escherichia coli strain FDAARGOS_1257

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: I6J87_RS08240
• Protein ID: WP_000132614.1
• Coordinates: 1695268..1695609 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	1695197..1695273 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J87_RS08230	1691872..1693341	+	1470	WP_001540814.1	type I restriction-modification system subunit M	-
I6J87_RS08235	1693341..1695047	+	1707	WP_001100194.1	restriction endonuclease subunit S	-
-	1695197..1695273	-	77	NuclAT_6	-	Antitoxin
-	1695197..1695273	-	77	NuclAT_6	-	Antitoxin
-	1695197..1695273	-	77	NuclAT_6	-	Antitoxin
-	1695197..1695273	-	77	NuclAT_6	-	Antitoxin
-	1695197..1695273	-	77	NuclAT_7	-	Antitoxin
-	1695197..1695273	-	77	NuclAT_7	-	Antitoxin
-	1695197..1695273	-	77	NuclAT_7	-	Antitoxin
-	1695197..1695273	-	77	NuclAT_7	-	Antitoxin
I6J87_RS08240	1695268..1695609	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
I6J87_RS08245	1695656..1697749	-	2094	WP_000648237.1	DUF262 domain-containing protein	-
I6J87_RS08250	1697847..1697927	-	81	WP_020233658.1	hypothetical protein	-
I6J87_RS08255	1698155..1699075	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
I6J87_RS08260	1699260..1700540	+	1281	WP_001298033.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	1679076..1697812	18736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T190589 WP_000132614.1 NZ_CP069480:1695268-1695609 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T190589 NZ_CP091481:31815-32129 [Enterobacter cloacae complex sp. ECL414]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCACGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT190589 NZ_CP069480:c1695273-1695197 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin