Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1000615..1001294 Replicon chromosome
Accession NZ_CP069480
Organism Escherichia coli strain FDAARGOS_1257

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag I6J87_RS04955 Protein ID WP_000057523.1
Coordinates 1000992..1001294 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6J87_RS04950 Protein ID WP_000806442.1
Coordinates 1000615..1000956 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J87_RS04940 996859..997791 - 933 WP_000883025.1 glutaminase A -
I6J87_RS04945 998053..1000557 + 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
I6J87_RS04950 1000615..1000956 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
I6J87_RS04955 1000992..1001294 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J87_RS04960 1001427..1002221 + 795 WP_000365148.1 TraB/GumN family protein -
I6J87_RS04965 1002425..1002904 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6J87_RS04970 1002928..1003728 + 801 WP_000439798.1 hypothetical protein -
I6J87_RS04975 1003725..1004228 + 504 WP_000667000.1 hypothetical protein -
I6J87_RS04980 1004266..1005918 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T190582 WP_000057523.1 NZ_CP069480:c1001294-1000992 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T190582 NZ_CP091470:2216801-2216904 [Klebsiella michiganensis]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT190582 WP_000806442.1 NZ_CP069480:c1000956-1000615 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT190582 NZ_CP091470:c2216911-2216743 [Klebsiella michiganensis]
AACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGCGACAAAAGTG
GACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References