Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 56735..57297 Replicon chromosome
Accession NZ_CP069480
Organism Escherichia coli strain FDAARGOS_1257

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6J87_RS00300 Protein ID WP_000605675.1
Coordinates 56735..57013 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6J87_RS00305 Protein ID WP_000781370.1
Coordinates 57013..57297 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J87_RS00275 52321..52752 - 432 WP_000152305.1 peroxiredoxin OsmC -
I6J87_RS00280 53097..53312 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6J87_RS00285 53414..53551 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6J87_RS00290 53708..55405 + 1698 WP_000433462.1 malate dehydrogenase -
I6J87_RS00295 55539..56549 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
I6J87_RS00300 56735..57013 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J87_RS00305 57013..57297 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
I6J87_RS00310 57526..58179 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6J87_RS00315 58172..59056 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
I6J87_RS00320 59069..62116 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T190581 WP_000605675.1 NZ_CP069480:56735-57013 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T190581 NZ_CP091470:2031099-2031431 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCAGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGCGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTCCACTGGAAGGGGCAGGGATAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAACGCATACCAGACGCGGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTACGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT190581 WP_000781370.1 NZ_CP069480:57013-57297 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT190581 NZ_CP091470:2030841-2031098 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References