Detailed information of TA system TA190558

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62111..62365
• Replicon: plasmid unnamed2
• Accession: NZ_CP069461
• Organism: Escherichia coli strain FDAARGOS_1249

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: I6J79_RS24965
• Protein ID: WP_001362723.1
• Coordinates: 62159..62365 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	62111..62172 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J79_RS24940	59286..60032	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
I6J79_RS24945	60087..60647	+	561	WP_000139325.1	fertility inhibition protein FinO	-
I6J79_RS24950	60779..60991	+	213	WP_001301409.1	ANR family transcriptional regulator	-
I6J79_RS24955	61237..61323	+	87	Protein_75	endonuclease	-
I6J79_RS24960	61504..61815	-	312	WP_000802277.1	hypothetical protein	-
-	62111..62172	-	62	NuclAT_1	-	Antitoxin
-	62111..62172	-	62	NuclAT_1	-	Antitoxin
-	62111..62172	-	62	NuclAT_1	-	Antitoxin
-	62111..62172	-	62	NuclAT_1	-	Antitoxin
I6J79_RS24965	62159..62365	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6J79_RS24970	62649..62906	+	258	WP_000083845.1	replication regulatory protein RepA	-
I6J79_RS24975	63141..63215	+	75	WP_057113837.1	RepA leader peptide Tap	-
I6J79_RS24980	63208..63804	+	597	Protein_80	incFII family plasmid replication initiator RepA	-
I6J79_RS24985	63856..64560	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I6J79_RS24990	64624..64728	+	105	Protein_82	incFII family plasmid replication initiator RepA	-
I6J79_RS24995	65648..65956	-	309	Protein_83	transposase	-
I6J79_RS25000	66149..66247	+	99	WP_172400466.1	transposase	-
I6J79_RS25005	66397..66645	-	249	WP_000738203.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	senB	1..114299	114299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T190558 WP_001362723.1 NZ_CP069461:62159-62365 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T190558 NZ_CP091450:c1585696-1585517 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACTTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT190558 NZ_CP069461:c62172-62111 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin