Detailed information of TA system TA190555

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37284..37553
• Replicon: plasmid unnamed2
• Accession: NZ_CP069461
• Organism: Escherichia coli strain FDAARGOS_1249

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J79_RS24825
• Protein ID: WP_001312861.1
• Coordinates: 37395..37553 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37284..37349 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J79_RS24795	33053..33592	+	540	WP_001530505.1	single-stranded DNA-binding protein	-
I6J79_RS24800	33654..33887	+	234	WP_000006030.1	DUF905 family protein	-
I6J79_RS24805	33952..35910	+	1959	WP_204147837.1	ParB/RepB/Spo0J family partition protein	-
I6J79_RS24810	35965..36399	+	435	WP_204147838.1	conjugation system SOS inhibitor PsiB	-
I6J79_RS24815	36396..37115	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
I6J79_RS24820	37127..37315	-	189	WP_001336239.1	hypothetical protein	-
-	37127..37351	+	225	NuclAT_0	-	-
-	37127..37351	+	225	NuclAT_0	-	-
-	37127..37351	+	225	NuclAT_0	-	-
-	37127..37351	+	225	NuclAT_0	-	-
-	37284..37349	-	66	-	-	Antitoxin
I6J79_RS24825	37395..37553	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6J79_RS24830	38001..38198	-	198	Protein_50	hypothetical protein	-
I6J79_RS24835	38200..38487	+	288	WP_000107535.1	hypothetical protein	-
I6J79_RS24840	38608..38943	+	336	Protein_52	DUF932 domain-containing protein	-
I6J79_RS24845	38987..39211	-	225	Protein_53	transposase	-
I6J79_RS24850	39270..40238	-	969	WP_073970534.1	IS5 family transposase	-
I6J79_RS24855	40269..41266	-	998	Protein_55	IS3 family transposase	-
I6J79_RS24860	41321..42055	+	735	Protein_56	conjugal transfer protein TraN	-
I6J79_RS24865	42082..42339	+	258	WP_000864309.1	conjugal transfer protein TrbE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	senB	1..114299	114299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190555 WP_001312861.1 NZ_CP069461:37395-37553 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190555 NZ_CP091430:1239021-1239371 [Paenibacillus sp. PHS-Z3]
TTGATCGTTAAACGCGGCGATGTGTTTTTTGCGGACTTATCCCCGGTCGTCGGTTCGGAGCAGGGGGGCGTTCGCCCGGT
ACTGGTCATTCAGAATGATATCGGTAACAGGTTCAGTCCGACGGTGATCGTGGCTGCCATAACGGCGCAAATTCAGAAGG
CCAAACTGCCGACTCATGTTGAGATCGATGCGGCTCTGCACGGTTTCGACCGCGACTCGGTGCTTCTTCTGGAGCAGATT
CGAACGATCGATAAACAGCGGCTTACCGATAAAATTACGCATTTGGACGACGAAACGATGCGTAAAGTGGATGAAGCTCT
TCAAATAAGCGTTGGGCTAATCGATTTCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT190555 NZ_CP069461:c37349-37284 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin