Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 37127..37553 Replicon plasmid unnamed2
Accession NZ_CP069461
Organism Escherichia coli strain FDAARGOS_1249

Toxin (Protein)


Gene name hok Uniprot ID P11895
Locus tag I6J79_RS24825 Protein ID WP_001312861.1
Coordinates 37395..37553 (+) Length 53 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 37127..37351 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J79_RS24790 32182..32410 + 229 Protein_42 hypothetical protein -
I6J79_RS24795 33053..33592 + 540 WP_001530505.1 single-stranded DNA-binding protein -
I6J79_RS24800 33654..33887 + 234 WP_000006030.1 DUF905 family protein -
I6J79_RS24805 33952..35910 + 1959 WP_204147837.1 ParB/RepB/Spo0J family partition protein -
I6J79_RS24810 35965..36399 + 435 WP_204147838.1 conjugation system SOS inhibitor PsiB -
I6J79_RS24815 36396..37115 + 720 WP_001276234.1 plasmid SOS inhibition protein A -
I6J79_RS24820 37127..37315 - 189 WP_001336239.1 hypothetical protein -
- 37127..37351 + 225 NuclAT_0 - Antitoxin
- 37127..37351 + 225 NuclAT_0 - Antitoxin
- 37127..37351 + 225 NuclAT_0 - Antitoxin
- 37127..37351 + 225 NuclAT_0 - Antitoxin
I6J79_RS24825 37395..37553 + 159 WP_001312861.1 type I toxin-antitoxin system Hok family toxin Toxin
I6J79_RS24830 38001..38198 - 198 Protein_50 hypothetical protein -
I6J79_RS24835 38200..38487 + 288 WP_000107535.1 hypothetical protein -
I6J79_RS24840 38608..38943 + 336 Protein_52 DUF932 domain-containing protein -
I6J79_RS24845 38987..39211 - 225 Protein_53 transposase -
I6J79_RS24850 39270..40238 - 969 WP_073970534.1 IS5 family transposase -
I6J79_RS24855 40269..41266 - 998 Protein_55 IS3 family transposase -
I6J79_RS24860 41321..42055 + 735 Protein_56 conjugal transfer protein TraN -
I6J79_RS24865 42082..42339 + 258 WP_000864309.1 conjugal transfer protein TrbE -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - senB 1..114299 114299


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-48)


Sequences


Toxin        


Download         Length: 53 a.a.        Molecular weight: 6082.32 Da        Isoelectric Point: 9.2584

>T190554 WP_001312861.1 NZ_CP069461:37395-37553 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Download         Length: 159 bp

>T190554 NZ_CP091428:123172-123588 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 225 bp

>AT190554 NZ_CP069461:37127-37351 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGTCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB P11895


Antitoxin

Download structure file

References