Detailed information of TA system TA190550

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 33934..34187
• Replicon: plasmid unnamed1
• Accession: NZ_CP069460
• Organism: Escherichia coli strain FDAARGOS_1249

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: I6J79_RS24560
• Protein ID: WP_001336447.1
• Coordinates: 34038..34187 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	33934..33990 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J79_RS24535	29846..30592	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
I6J79_RS24540	30647..31204	+	558	WP_204147836.1	fertility inhibition protein FinO	-
I6J79_RS24545	32301..32762	+	462	WP_000760080.1	thermonuclease family protein	-
I6J79_RS24550	32865..33249	+	385	Protein_30	hypothetical protein	-
I6J79_RS24555	33402..33839	+	438	WP_000872609.1	hypothetical protein	-
-	33934..33990	-	57	NuclAT_0	-	Antitoxin
-	33934..33990	-	57	NuclAT_0	-	Antitoxin
-	33934..33990	-	57	NuclAT_0	-	Antitoxin
-	33934..33990	-	57	NuclAT_0	-	Antitoxin
I6J79_RS24560	34038..34187	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6J79_RS24565	34472..34729	+	258	WP_000084404.1	replication regulatory protein RepA	-
I6J79_RS24570	34964..35038	+	75	WP_001365705.1	RepA leader peptide Tap	-
I6J79_RS24575	35031..35888	+	858	WP_016239092.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36471	36471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T190550 WP_001336447.1 NZ_CP069460:34038-34187 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T190550 NZ_CP091427:3998243-3998707 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT190550 NZ_CP069460:c33990-33934 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin