Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4118925..4119724 Replicon chromosome
Accession NZ_CP069459
Organism Escherichia coli strain FDAARGOS_1249

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag I6J79_RS20010 Protein ID WP_000347275.1
Coordinates 4118925..4119389 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag I6J79_RS20015 Protein ID WP_001307405.1
Coordinates 4119389..4119724 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J79_RS19980 4113926..4114360 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
I6J79_RS19985 4114378..4115256 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
I6J79_RS19990 4115246..4116025 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
I6J79_RS19995 4116036..4116509 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6J79_RS20000 4116532..4117812 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6J79_RS20005 4118061..4118870 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
I6J79_RS20010 4118925..4119389 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6J79_RS20015 4119389..4119724 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6J79_RS20020 4119873..4121444 - 1572 WP_001273753.1 galactarate dehydratase -
I6J79_RS20025 4121819..4123153 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
I6J79_RS20030 4123169..4123939 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T190543 WP_000347275.1 NZ_CP069459:c4119389-4118925 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T190543 NZ_CP091427:c2755085-2754831 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT190543 WP_001307405.1 NZ_CP069459:c4119724-4119389 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT190543 NZ_CP091427:c2755333-2755082 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References