Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2401063..2401757 Replicon chromosome
Accession NZ_CP069459
Organism Escherichia coli strain FDAARGOS_1249

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag I6J79_RS11950 Protein ID WP_001263488.1
Coordinates 2401063..2401461 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag I6J79_RS11955 Protein ID WP_000554758.1
Coordinates 2401464..2401757 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 2396652..2396732 - 81 NuclAT_12 - -
- 2396652..2396732 - 81 NuclAT_12 - -
- 2396652..2396732 - 81 NuclAT_12 - -
- 2396652..2396732 - 81 NuclAT_12 - -
I6J79_RS11925 2397328..2397786 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
I6J79_RS11930 2398047..2399504 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
I6J79_RS11935 2399561..2400082 - 522 Protein_2344 peptide chain release factor H -
I6J79_RS11940 2400078..2400284 - 207 Protein_2345 RtcB family protein -
I6J79_RS11945 2400601..2401053 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
I6J79_RS11950 2401063..2401461 - 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
I6J79_RS11955 2401464..2401757 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
I6J79_RS11960 2401809..2402864 - 1056 WP_001226164.1 DNA polymerase IV -
I6J79_RS11965 2402935..2403720 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
I6J79_RS11970 2403692..2405404 + 1713 Protein_2351 flagellar biosynthesis protein FlhA -
I6J79_RS11975 2405628..2406125 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2401063..2416343 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T190540 WP_001263488.1 NZ_CP069459:c2401461-2401063 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T190540 NZ_CP091427:2136012-2136188 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT190540 WP_000554758.1 NZ_CP069459:c2401757-2401464 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT190540 NZ_CP091427:2136234-2136650 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References