Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 351674..352176 Replicon chromosome
Accession NZ_CP069459
Organism Escherichia coli strain FDAARGOS_1249

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag I6J79_RS01620 Protein ID WP_000767829.1
Coordinates 351922..352176 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6J79_RS01615 Protein ID WP_001259255.1
Coordinates 351674..351925 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J79_RS01590 346852..347919 - 1068 WP_000080135.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I6J79_RS01595 347919..348989 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
I6J79_RS01600 348992..350290 - 1299 WP_000009595.1 histidinol dehydrogenase -
I6J79_RS01605 350296..351195 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6J79_RS01610 351341..351391 - 51 WP_001364200.1 his operon leader peptide -
I6J79_RS01615 351674..351925 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6J79_RS01620 351922..352176 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6J79_RS01625 352259..353083 + 825 WP_000754737.1 SDR family oxidoreductase -
I6J79_RS01630 353129..354058 + 930 WP_000803351.1 LysR family transcriptional regulator -
I6J79_RS01635 354273..354335 + 63 WP_010723108.1 membrane protein YoeI -
I6J79_RS01640 354325..355683 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
I6J79_RS01645 355862..356920 + 1059 WP_000492339.1 transport protein YeeE -
I6J79_RS01650 356934..357161 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T190527 WP_000767829.1 NZ_CP069459:351922-352176 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T190527 NZ_CP091425:c210675-210337 [Methylobacter sp. YRD-M1]
ATGGCTGCGACGTATATACCCGAACGCGGCGATTTGGTTTGGATCGCGTTCGATCCGCAGGCAGGGCATGAACAAGCGGG
GCGCCGCCCCGGTTTGGTTATTACGCCACACGCTTATAACGCGAAAGCCGGGTTGGCGTTGATTTGCCCTGTGACAAGTC
AAGTTAAAGGCTATCCCTTCGAGGTCGCTTTACCGGTCGGCGGCCGGGTCGGTGGCGTGGTGTTGGCGGATCAGGTTCGT
AGTTTGGATTGGGCCGCGCGGCAAGCCGAAAAGGCATCTAAAGTGGATGGCAATGTGCTAGCCGAAGTGTTGGCAAAGAT
CGAAGCGCTCATTCAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT190527 WP_001259255.1 NZ_CP069459:351674-351925 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT190527 NZ_CP091425:c210923-210675 [Methylobacter sp. YRD-M1]
ATGCACACTGAAATTAAGAAATGGGGCAATAGCGCCGTCGTTCGATTACCGGCTTCGATGCTTGCGCGGTTAAAGCTGGA
AGTGGGTTCGCCGGTCGAGCTGACGGTTGACGACGCTGGTTTTCGAATTGTGCCCACGGCTGCCAAGCCTGTATTCAAGC
TAGATGATTTACTGGCTGGCATTACTGCCGATAACCGTCATGATTCTATTGAGTGGGGTCCAGACGTGGGCCGCGAGAGT
ACATCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References