Detailed information of TA system TA190525

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4014..4283
• Replicon: plasmid unnamed3
• Accession: NZ_CP069456
• Organism: Escherichia coli strain FDAARGOS_1254

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J84_RS23200
• Protein ID: WP_001312861.1
• Coordinates: 4125..4283 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	4014..4079 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J84_RS23175	368..601	+	234	WP_000006003.1	DUF905 family protein	-
I6J84_RS23180	662..2626	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
I6J84_RS23185	2695..3129	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6J84_RS23190	3126..3845	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	3857..4081	+	225	NuclAT_0	-	-
-	3857..4081	+	225	NuclAT_0	-	-
-	3857..4081	+	225	NuclAT_0	-	-
-	3857..4081	+	225	NuclAT_0	-	-
-	3860..4081	-	222	NuclAT_0	-	-
I6J84_RS23195	3866..4045	-	180	WP_001309233.1	hypothetical protein	-
-	4014..4079	+	66	NuclAT_1	-	-
I6J84_RS23200	4125..4283	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6J84_RS23205	5199..5495	+	297	WP_001272251.1	hypothetical protein	-
I6J84_RS23210	5606..6427	+	822	WP_021549731.1	DUF945 domain-containing protein	-
I6J84_RS23215	6724..7371	-	648	Protein_9	transglycosylase SLT domain-containing protein	-
I6J84_RS23220	7647..8030	+	384	WP_001151529.1	relaxosome protein TraM	-
I6J84_RS23225	8222..8869	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
I6J84_RS23230	8977..9216	+	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..68946	68946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190525 WP_001312861.1 NZ_CP069456:4125-4283 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190525 NZ_CP091424:995147-995518 [Methylobacter sp. YRD-M1]
ATGATATTAGTAGATACGTCGGTCTGGATAGATCACTTGCGACGCAAAGATGCTGAGTTGGCCGCTATGTTGTCGGAGGG
ACAAGTACTGGTGCATCCCTGGATAATAGGCGAATTGGCATGCGGTAATTTGCAAGACCGCAAACTGGTGCTGGATTTAT
TGCAGTCATTGCCCTCGGCATCTGTAGCGAGTACGGGCGAGGTGCTGTTTTTCATTGAAAAGCATGTCTTGATGGGGCGA
GGTATCGGCTACGTAGATGTGCATCTGTTGGCTGCCGCCCGCCTGGCAGGAACGCGTCTTTGGACACGGGATAAGCGACT
GGTCGTTGTCGCCGATGAACTCGGTATGGCCTATACGGAAACTCGGCACTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT190525 NZ_CP069456:c4079-4014 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin