Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2849055..2849734 Replicon chromosome
Accession NZ_CP069453
Organism Escherichia coli strain FDAARGOS_1254

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag I6J84_RS13275 Protein ID WP_000057524.1
Coordinates 2849055..2849357 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag I6J84_RS13280 Protein ID WP_000806442.1
Coordinates 2849393..2849734 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J84_RS13250 2844431..2846083 + 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
I6J84_RS13255 2846121..2846624 - 504 WP_000667000.1 hypothetical protein -
I6J84_RS13260 2846621..2847421 - 801 WP_000439798.1 hypothetical protein -
I6J84_RS13265 2847445..2847924 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
I6J84_RS13270 2848128..2848922 - 795 WP_000365177.1 TraB/GumN family protein -
I6J84_RS13275 2849055..2849357 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J84_RS13280 2849393..2849734 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
I6J84_RS13285 2849792..2852296 - 2505 WP_000084000.1 copper-exporting P-type ATPase CopA -
I6J84_RS13290 2852559..2853491 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T190506 WP_000057524.1 NZ_CP069453:2849055-2849357 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T190506 NZ_CP091412:412010-412219 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGTATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT190506 WP_000806442.1 NZ_CP069453:2849393-2849734 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT190506 NZ_CP091412:411705-411998 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References