Detailed information of TA system TA190504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2621190..2621884
• Replicon: chromosome
• Accession: NZ_CP069453
• Organism: Escherichia coli strain FDAARGOS_1254

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6J84_RS12230
• Protein ID: WP_001263500.1
• Coordinates: 2621486..2621884 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6J84_RS12225
• Protein ID: WP_000554758.1
• Coordinates: 2621190..2621483 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J84_RS12205	2616926..2617423	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
I6J84_RS12210	2617543..2619255	-	1713	Protein_2362	flagellar biosynthesis protein FlhA	-
I6J84_RS12215	2619227..2620012	+	786	WP_000207543.1	putative lateral flagellar export/assembly protein LafU	-
I6J84_RS12220	2620083..2621138	+	1056	WP_001226162.1	DNA polymerase IV	-
I6J84_RS12225	2621190..2621483	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6J84_RS12230	2621486..2621884	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6J84_RS12235	2621894..2622346	+	453	WP_001059897.1	GNAT family N-acetyltransferase	-
I6J84_RS12240	2622536..2623675	+	1140	WP_000521564.1	RNA ligase RtcB family protein	-
I6J84_RS12245	2623672..2624286	+	615	WP_000602123.1	peptide chain release factor H	-
I6J84_RS12250	2624343..2625800	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6J84_RS12255	2626061..2626519	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2590912..2622346	31434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T190504 WP_001263500.1 NZ_CP069453:2621486-2621884 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T190504 NZ_CP091412:19731-19907 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAACAAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT190504 WP_000554758.1 NZ_CP069453:2621190-2621483 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT190504 NZ_CP091412:c20069-19895 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGACTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTGTTG

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3