Detailed information of TA system TA190488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4536422..4537221
• Replicon: chromosome
• Accession: NZ_CP069451
• Organism: Escherichia coli strain FDAARGOS_1256

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: I6J86_RS21475
• Protein ID: WP_000347252.1
• Coordinates: 4536757..4537221 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: I6J86_RS21470
• Protein ID: WP_001296435.1
• Coordinates: 4536422..4536757 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J86_RS21455	4532207..4532977	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
I6J86_RS21460	4532993..4534327	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
I6J86_RS21465	4534702..4536273	+	1572	WP_001273939.1	galactarate dehydratase	-
I6J86_RS21470	4536422..4536757	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I6J86_RS21475	4536757..4537221	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I6J86_RS21480	4537276..4538085	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I6J86_RS21485	4538334..4539614	+	1281	WP_000681930.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I6J86_RS21490	4539637..4540110	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I6J86_RS21495	4540121..4540900	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
I6J86_RS21500	4540890..4541768	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
I6J86_RS21505	4541786..4542220	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T190488 WP_000347252.1 NZ_CP069451:4536757-4537221 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T190488 NZ_CP091369:3978876-3979265 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTACTTTGAAGATCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT190488 WP_001296435.1 NZ_CP069451:4536422-4536757 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT190488 NZ_CP091369:3978612-3978869 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5