Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2557094..2557656 Replicon chromosome
Accession NZ_CP069451
Organism Escherichia coli strain FDAARGOS_1256

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6J86_RS12210 Protein ID WP_000605675.1
Coordinates 2557378..2557656 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6J86_RS12205 Protein ID WP_000781370.1
Coordinates 2557094..2557378 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J86_RS12190 2552275..2555322 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
I6J86_RS12195 2555335..2556219 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
I6J86_RS12200 2556212..2556865 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6J86_RS12205 2557094..2557378 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
I6J86_RS12210 2557378..2557656 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J86_RS12215 2557842..2558852 - 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
I6J86_RS12220 2558986..2560683 - 1698 WP_000433462.1 malate dehydrogenase -
I6J86_RS12225 2560840..2560977 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6J86_RS12230 2561079..2561294 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6J86_RS12235 2561639..2562070 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T190473 WP_000605675.1 NZ_CP069451:c2557656-2557378 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T190473 NZ_CP091361:c1023006-1022824 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT190473 WP_000781370.1 NZ_CP069451:c2557378-2557094 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT190473 NZ_CP091361:c1022731-1022327 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References