Detailed information of TA system TA190469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1300792..1301486
• Replicon: chromosome
• Accession: NZ_CP069451
• Organism: Escherichia coli strain FDAARGOS_1256

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: I6J86_RS06120
• Protein ID: WP_204159020.1
• Coordinates: 1301088..1301486 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6J86_RS06115
• Protein ID: WP_000554758.1
• Coordinates: 1300792..1301085 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J86_RS06090	1296328..1296825	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
I6J86_RS06095	1296908..1297117	-	210	WP_071778446.1	hypothetical protein	-
I6J86_RS06100	1297145..1298857	-	1713	Protein_1176	flagellar biosynthesis protein FlhA	-
I6J86_RS06105	1298829..1299614	+	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
I6J86_RS06110	1299685..1300740	+	1056	WP_001226168.1	DNA polymerase IV	-
I6J86_RS06115	1300792..1301085	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6J86_RS06120	1301088..1301486	+	399	WP_204159020.1	type II toxin-antitoxin system YafO family toxin	Toxin
I6J86_RS06125	1301496..1301948	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
I6J86_RS06130	1302138..1303277	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
I6J86_RS06135	1303274..1303888	+	615	WP_000602124.1	peptide chain release factor H	-
I6J86_RS06140	1303945..1305402	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6J86_RS06145	1305663..1306121	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	1281267..1301948	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15397.89 Da
• Isoelectric Point: 9.5493

>T190469 WP_204159020.1 NZ_CP069451:1301088-1301486 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTAVFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T190469 NZ_CP091361:c956482-956195 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT190469 WP_000554758.1 NZ_CP069451:1300792-1301085 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT190469 NZ_CP091361:c956208-955894 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: S1QAE3