Detailed information of TA system TA190462

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 907460..907872
• Replicon: chromosome
• Accession: NZ_CP069451
• Organism: Escherichia coli strain FDAARGOS_1256

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: I6J86_RS04230
• Protein ID: WP_000132614.1
• Coordinates: 907460..907801 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	907796..907872 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J86_RS04210	902529..903809	-	1281	WP_001298033.1	DUF445 domain-containing protein	-
I6J86_RS04215	903994..904914	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
I6J86_RS04220	905142..905222	+	81	WP_020233658.1	hypothetical protein	-
I6J86_RS04225	905320..907413	+	2094	WP_000648237.1	DUF262 domain-containing protein	-
I6J86_RS04230	907460..907801	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
-	907796..907872	+	77	NuclAT_6	-	Antitoxin
-	907796..907872	+	77	NuclAT_6	-	Antitoxin
-	907796..907872	+	77	NuclAT_6	-	Antitoxin
-	907796..907872	+	77	NuclAT_6	-	Antitoxin
-	907796..907872	+	77	NuclAT_7	-	Antitoxin
-	907796..907872	+	77	NuclAT_7	-	Antitoxin
-	907796..907872	+	77	NuclAT_7	-	Antitoxin
-	907796..907872	+	77	NuclAT_7	-	Antitoxin
I6J86_RS04235	908022..909728	-	1707	WP_001100194.1	restriction endonuclease subunit S	-
I6J86_RS04240	909728..911197	-	1470	WP_001540814.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T190462 WP_000132614.1 NZ_CP069451:c907801-907460 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T190462 NZ_CP091357:3830682-3830864 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT190462 NZ_CP069451:907796-907872 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin