Detailed information of TA system TA190459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 298418..299020
• Replicon: chromosome
• Accession: NZ_CP069451
• Organism: Escherichia coli strain FDAARGOS_1256

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6J86_RS01425
• Protein ID: WP_000897305.1
• Coordinates: 298418..298729 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J86_RS01430
• Protein ID: WP_000356397.1
• Coordinates: 298730..299020 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J86_RS01400	294332..294931	+	600	WP_001298594.1	glucose-1-phosphatase	-
I6J86_RS01405	294925..295797	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J86_RS01410	295794..296231	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J86_RS01415	296276..297217	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J86_RS01420	297281..298189	-	909	WP_001298596.1	alpha/beta hydrolase	-
I6J86_RS01425	298418..298729	+	312	WP_000897305.1	hypothetical protein	Toxin
I6J86_RS01430	298730..299020	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J86_RS01435	299625..299843	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
I6J86_RS01440	300058..300987	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6J86_RS01445	300984..301619	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6J86_RS01450	301616..302518	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T190459 WP_000897305.1 NZ_CP069451:298418-298729 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T190459 NZ_CP091356:c3014604-3014302 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT190459 WP_000356397.1 NZ_CP069451:298730-299020 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT190459 NZ_CP091356:c3014301-3014020 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7