Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2229816..2230318 Replicon chromosome
Accession NZ_CP069447
Organism Escherichia coli strain FDAARGOS_1255

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I6J85_RS10435 Protein ID WP_000767822.1
Coordinates 2230064..2230318 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I6J85_RS10430 Protein ID WP_001259255.1
Coordinates 2229816..2230067 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J85_RS10405 2224994..2226061 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
I6J85_RS10410 2226061..2227131 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
I6J85_RS10415 2227128..2228432 - 1305 WP_001297912.1 histidinol dehydrogenase -
I6J85_RS10420 2228438..2229337 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
I6J85_RS10425 2229483..2229533 - 51 WP_001364200.1 his operon leader peptide -
I6J85_RS10430 2229816..2230067 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I6J85_RS10435 2230064..2230318 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I6J85_RS10440 2230401..2231225 + 825 WP_000754733.1 SDR family oxidoreductase -
I6J85_RS10445 2231271..2232200 + 930 WP_000803366.1 LysR family transcriptional regulator -
I6J85_RS10450 2232415..2232477 + 63 WP_010723108.1 membrane protein YoeI -
I6J85_RS10455 2232467..2233825 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T190437 WP_000767822.1 NZ_CP069447:2230064-2230318 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T190437 NZ_CP091345:c2641257-2641075 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT190437 WP_001259255.1 NZ_CP069447:2229816-2230067 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT190437 NZ_CP091345:c2641010-2640606 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References