190436

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1523531..1524258 Replicon chromosome
Accession NZ_CP069447
Organism Escherichia coli strain FDAARGOS_1255

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag I6J85_RS07240 Protein ID WP_000547555.1
Coordinates 1523531..1523842 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag I6J85_RS07245 Protein ID WP_000126293.1
Coordinates 1523839..1524258 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J85_RS07210 1518672..1520381 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
I6J85_RS07215 1520391..1520933 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
I6J85_RS07220 1520933..1521700 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
I6J85_RS07225 1521697..1522107 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
I6J85_RS07230 1522100..1522570 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
I6J85_RS07235 1522613..1523368 + 756 WP_001305268.1 hypothetical protein -
I6J85_RS07240 1523531..1523842 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
I6J85_RS07245 1523839..1524258 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
I6J85_RS07250 1524350..1524778 - 429 WP_000536064.1 hypothetical protein -
I6J85_RS07255 1525163..1525690 + 528 WP_001078777.1 electron transport protein HydN -
I6J85_RS07260 1525843..1528095 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T190436 WP_000547555.1 NZ_CP069447:1523531-1523842 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T190436 NZ_CP091344:3123463-3123852 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT190436 WP_000126293.1 NZ_CP069447:1523839-1524258 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT190436 NZ_CP091344:3123199-3123456 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References