Detailed information of TA system TA190433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 986422..987221
• Replicon: chromosome
• Accession: NZ_CP069447
• Organism: Escherichia coli strain FDAARGOS_1255

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: I6J85_RS04745
• Protein ID: WP_000347252.1
• Coordinates: 986422..986886 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: I6J85_RS04750
• Protein ID: WP_001296435.1
• Coordinates: 986886..987221 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J85_RS04715	981423..981857	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
I6J85_RS04720	981875..982753	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
I6J85_RS04725	982743..983522	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
I6J85_RS04730	983533..984006	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I6J85_RS04735	984029..985309	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I6J85_RS04740	985558..986367	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I6J85_RS04745	986422..986886	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I6J85_RS04750	986886..987221	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I6J85_RS04755	987370..988941	-	1572	WP_001273940.1	galactarate dehydratase	-
I6J85_RS04760	989316..990650	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
I6J85_RS04765	990666..991436	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T190433 WP_000347252.1 NZ_CP069447:c986886-986422 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T190433 NZ_CP091342:c1154070-1153681 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT190433 WP_001296435.1 NZ_CP069447:c987221-986886 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT190433 NZ_CP091342:c1154334-1154077 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5