Detailed information of TA system TA190431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 128261..128863
• Replicon: chromosome
• Accession: NZ_CP069447
• Organism: Escherichia coli strain FDAARGOS_1255

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6J85_RS00580
• Protein ID: WP_000897302.1
• Coordinates: 128552..128863 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J85_RS00575
• Protein ID: WP_000356397.1
• Coordinates: 128261..128551 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J85_RS00550	124334..125236	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
I6J85_RS00555	125233..125868	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6J85_RS00560	125865..126794	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6J85_RS00565	127010..127228	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
I6J85_RS00570	127624..127902	-	279	WP_001296612.1	hypothetical protein	-
I6J85_RS00575	128261..128551	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J85_RS00580	128552..128863	-	312	WP_000897302.1	hypothetical protein	Toxin
I6J85_RS00585	129093..130001	+	909	WP_001305059.1	alpha/beta hydrolase	-
I6J85_RS00590	130065..131006	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J85_RS00595	131051..131488	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J85_RS00600	131485..132357	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J85_RS00605	132351..132950	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T190431 WP_000897302.1 NZ_CP069447:c128863-128552 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T190431 NZ_CP091341:c288588-288199 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT190431 WP_000356397.1 NZ_CP069447:c128551-128261 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT190431 NZ_CP091341:c288852-288595 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: S1P416