Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4672717..4673411 Replicon chromosome
Accession NZ_CP069445
Organism Escherichia coli strain FDAARGOS_1251

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag I6J81_RS22130 Protein ID WP_001263501.1
Coordinates 4673013..4673411 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag I6J81_RS22125 Protein ID WP_000554755.1
Coordinates 4672717..4673010 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J81_RS22100 4667769..4668062 + 294 WP_000896590.1 hypothetical protein -
I6J81_RS22105 4668431..4669003 + 573 WP_000086540.1 recombinase family protein -
I6J81_RS22110 4669007..4669969 - 963 WP_000231497.1 hypothetical protein -
I6J81_RS22115 4670007..4671551 - 1545 WP_000758648.1 recombinase family protein -
I6J81_RS22120 4671613..4672665 + 1053 WP_000691316.1 DNA polymerase IV -
I6J81_RS22125 4672717..4673010 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
I6J81_RS22130 4673013..4673411 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
I6J81_RS22135 4673421..4673873 + 453 WP_001059897.1 GNAT family N-acetyltransferase -
I6J81_RS22140 4674063..4675202 + 1140 WP_000521578.1 RNA ligase RtcB family protein -
I6J81_RS22145 4675199..4675813 + 615 WP_000602123.1 peptide chain release factor H -
I6J81_RS22150 4675870..4677327 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
I6J81_RS22155 4677588..4678046 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T190428 WP_001263501.1 NZ_CP069445:4673013-4673411 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T190428 NZ_CP091338:c2195698-2195309 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCACGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGTAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT190428 WP_000554755.1 NZ_CP069445:4672717-4673010 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT190428 NZ_CP091338:c2195962-2195705 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References