Detailed information of TA system TA190421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3638171..3638773
• Replicon: chromosome
• Accession: NZ_CP069445
• Organism: Escherichia coli strain FDAARGOS_1251

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6J81_RS17335
• Protein ID: WP_000897302.1
• Coordinates: 3638171..3638482 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J81_RS17340
• Protein ID: WP_000356397.1
• Coordinates: 3638483..3638773 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J81_RS17310	3634085..3634684	+	600	WP_001314324.1	glucose-1-phosphatase	-
I6J81_RS17315	3634678..3635550	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J81_RS17320	3635547..3635984	+	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J81_RS17325	3636029..3636970	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J81_RS17330	3637034..3637942	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6J81_RS17335	3638171..3638482	+	312	WP_000897302.1	hypothetical protein	Toxin
I6J81_RS17340	3638483..3638773	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J81_RS17345	3639132..3639410	+	279	WP_001296612.1	hypothetical protein	-
I6J81_RS17350	3639806..3640024	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
I6J81_RS17355	3640240..3641169	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6J81_RS17360	3641166..3641801	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6J81_RS17365	3641798..3642700	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T190421 WP_000897302.1 NZ_CP069445:3638171-3638482 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T190421 NZ_CP091332:57869-58285 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT190421 WP_000356397.1 NZ_CP069445:3638483-3638773 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT190421 NZ_CP091332:57642-57872 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: S1P416